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Leaf Spot Fungicide





Farming Smarter Project

Timeline: 2013 – 2015

Project Lead: Randy Kutcher, University of Saskatchewan

Lethbridge Project Contact: Ken Coles

Collaborators: Michael Harding, Brooks; Kelly Turkington, Lacombe; Bill May, Indian Head


Diseases [leaf spots, rusts and fusarium head blight (FHB)] have greatly reduced wheat yield and quality in western Canada in recent years.  Wheat growers want to know the level of leaf spot control that can be expected with a fungicide application at the heading stage (FHB timing) of wheat, or in other words if a single fungicide application aimed at control of FHB will also provide effective leaf spot control.  This proposed project aims to answer this question. Normally, fungicides are applied at the seedling stage and/or at flag leaf emergence for control of leaf spot diseases and at head emergence (to early anthesis)  for FHB, but there is very little information on the potential benefits of fungicide applied at the FHB timing (early anthesis) for leaf spot control.  In addition to conventional fungicides, new tools to manage FHB, e.g. biofungicides have been shown to reduce FHB symptoms and DON accumulation in durum wheat.

This project will determine the efficacy of conventional fungicides and a biofungicide, to manage fusarium head blight and leaf spotting diseases.  The results will be used to develop effective fungicide strategies for wheat growers.  We propose to evaluate the effect of fungicide applications for fungal leaf and head diseases of wheat at two application timings (flag emergence and early anthesis) when applied singly or in tank-mix pairs. We also plan to evaluate a biofungicide that claims to have efficacy against FHB.

Jamie shows leaf disease on an untreated check

Jamie shows leaf disease on an untreated check


Evaluate the efficacy of applications of synthetic fungicides and a biofungicide for fungal leaf and head diseases of wheat at two application timings (flag emergence and anthesis).


Location: Lethbridge, Brooks, Lacombe, Saskatoon, Indian Head and Melfort

We grew the Canada Western Spring wheat variety ‘Carberry’ because it’s moderately susceptible to leaf spotting but resistant to RHB. Applications of fungicide consisted of a single application at flag-leaf stage, a single application during anthesis, and a dual application at both stages.

Fungicide treatment:

  • prothioconazole + tebuconazole (Prosaro at 800 mL ha-1 )
  • tebuconazole (Folicur 250 EW at 499 mL ha-1 140 , or at Lacombe, 2014, Folicur 432 F at 291 mL ha-1
  • subtilis (Serenade Optimum 500 g ha-1 141 )

All treatments were applied in 100 L ha-1 142 of water.

Disease severity was determined by assessing ten flag leaves and ten penultimate leaves from each plot using the Horsfall–Barratt scale. This is a scale of 1 -11. Ratings were then converted to percent leaf area is affected by disease. 

Fifty random spikes were assessed from each plot through the early stages of development for FHB severity.


The data collected from each site-year were classified into high disease locations, where leaf-spot disease severity was 40 per cent or greater in unsprayed checks, and low disease locations, where disease severity was less than 40 per cent. Each classification was analyzed separately.

High Disease Locations – Saskatoon, Melfort and Lacombe

The average leaf-spot disease severity of the unsprayed check was 73.2 per cent.

Differences in disease severity:

  • Anthesis application– 41 per cent
  • Flag-leaf application – 34.7 per cent
  • Dual application – 20.2 per cent

Synthetic fungicides reduced leaf-spot disease severity compared to the untreated control. Biological fungicide (B. subtilis) did not. Tebuconazole reduced the severity to 36.1 per cent. Prothioconazole and tebuconazole reduced it to 27.6 per cent. There were no differences in FHB among fungicide treatments.

Low Disease Locations – Lethbridge, Brooks, Saskatoon, and Indian Head

The average disease severity of the unsprayed check was 16.2 per cent which was reduced to 6.6 per cent with the application of fungicide.

Differences in disease severity:

  • Anthesis application – 7.6 per cent
  • Flag-leaf application – 7.1 per cent
  • Dual application – 5 per cent

Differences in leaf disease severity between flag-leaf and anthesis stage fungicide were only detected at high disease locations. They were also observed when fungicide was applied at flag-leaf or anthesis timings compared to the dual application treatment under both high and low disease severity situations. The study determines the best time to apply fungicide was between the flag leaf and medium milk growth stages.

Our results indicate there was no yield penalty when applying fungicide at the anthesis stage under high disease scenarios. However, there was no yield benefit to fungicide application when the level of leaf diseases are low. Disease severity was lower when fungicide was applied at flag leaf stage as opposed to anthesis. A single fungicide application at anthesis, in comparison to the flag-leaf stage, will give producers control over leaf spotting activity, maintain their yield and improve grain quality.

Crop Protection The Official Journal of the International Association for the Plant Protection Sciences published an article about this project.

  Fungicide application at anthesis of wheat in western Canada



It is important to manage FHB and leaf spot diseases in wheat to reduce their impact on yield and quality.

A single fungicide application at anthesis was the optimal timing for leaf-spot control, grain yield and grain quality.

The most effective treatment in our study was the dual application of fungicide, which reduced leaf disease severity from 73.2% in the unsprayed check to 20.2% at the high disease locations, and from 13.7% to 6.0% the low disease locations. Fungicide applied at flag leaf stage and again at anthesis (the dual treatment) reduced disease severity compared to a single application at either stage. Fungicide applied at both stages increased yield compared to a fungicide application flag leaf stage; however, the increase in yield over a single application at flag leaf stage was marginal and the limited yield increase compared to a single application at anthesis would not justify a second application economically.

Applying the same fungicide family twice per growing season is discouraged due to increased selection for fungicide insensitive pathogen isolates.

Differences were observed in leaf disease severity between prothioconazole + tebuconazole and tebuconazole alone; the former was 23.5% more effective in high-disease fields and 13.8% in low-disease fields, although no differences in yield were observed.






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